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A Practical Guide to Protein and Peptide Purification for MicrosequencingFrom Academic Press
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Why a Second Edition?
The Second Edition provides practical answers to the general question, "How can I obtain useful sequence information from my protein or peptide?" rather than the more specific question asked in the first edition, "How can I obtain the N-terminal sequence?" Important new methods include ways of dealing with blocked N termini, computer analysis of protein sequences, and the recent revolution in mass spectrometry.
- Mass spectrophotometric characterization of proteins and peptides
- N-terminal sequencing of proteins with blocked N termini
- Internal amino acid sequence analysis after protease digestion in-gel and on-blot
- Improved microscale peptide purification methods
- Computer analysis of protein sequences
- New protocols tested and refined through everyday use in authors' laboratories
- Updated reference chapter covering all aspects of protein microsequencing
- Published on: 2012-12-02
- Released on: 2012-12-02
- Format: Kindle eBook
Review
Reviews of the First Edition: "Like the evening network television news programs, this book gives a useful summary of the highlights (of protein chemistry) to enable the masses to go forth armed with enough information to survive in the world." --Cell
From the Back Cover
Why a Second Edition?
The second edition provides practical answers to the general question, "How can I obtain useful sequence information from my protein or peptide?" rather than the more specific question asked in the first edition, "How can I obtain the N-terminal sequence?" Important new methods include ways of dealing with blocked N termini, computer analysis of protein sequences, and the recent revolution in mass spectrometry.
Highlights of the Second Edition:
* Mass spectrophotometric characterization of proteins and peptides
* N-terminal sequencing of proteins with blocked N termini
* Internal amino acid sequence analysis after protease digestion in-gel and on-blot
* Improved microscale peptide purification methods
* Computer analysis of protein sequences
* New protocols tested and refined through everyday use in authors' laboratories
* Updated reference chapter covering all aspects of protein microsequencing
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